Application

Research CategoryApoptosis & Cancer

Research Sub CategoryCytokines & Cytokine Receptors

Anti-EphA7 Antibody, clone 2F3.1 is an antibody against EphA7 for use in Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Ephrin type-A receptor 7 (EC 2.7.10.1; UniProt Q15375; also known as EHK-3, EK11, EPH homology kinase 3, EPH-like kinase 11, hEK11, Receptor protein-tyrosine kinase HEK11, Tyrosine-protein kinase receptor EHK-3) is encoded by the EPHA7 (also known as EHK3, HEK11) gene (Gene ID 2045) in human. EphA7 belongs to the ephrin receptor family of receptor tyrosine kinases that are activated upon ephrin binding. Ephrin receptor signaling plays important roles in embryonic development, tissue boundaries formation, angiogenesis, stem cell differentiation, and cancer. There exist nine type-A (EphA1-8 and EphA10) and five type-B (EphB1-4 and EphB6) ephrin receptors in human. EphA7 is initially produced with a signal peptide (a.a. 1-27) sequence, the removal of which yields the mature protein with a large extracellular (a.a. 28-555) region, followed by a transmembrane segment (a.a. 556-576) and a cytoplasmic region (a.a. 577-998) that contains the kinase domain (a.a. 633-894).

Immunogen

GST-tagged recombinant extracellular N-terminal fragment of human EphA7.

Epitope: Extracellular domain.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G Purified

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blotting in MCF-7 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected EphA7 in 10 µg of MCF-7 cell lysate.

Specificity

Expected to react with all five spliced isoforms of human EphA7 reported by UniProt (Q15375).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~120 kDa observed. 112.1 kDa (isoform 1; EPHA7-FL), 111.5 kDa (isoform 2), 31.83 kDa (isoform 3), 111.6 kDa (isoform 4), and 50.52 kDa (isoform 5; EPHA7-S) calculated.